Two-step enzyme immunoassay for the determination of serum .ALPHA.-fetoprotein.
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چکیده
منابع مشابه
Two-site enzyme immunoassay for alpha-fetoprotein in dried-blood samples collected on filter paper.
This is a method for measuring alpha-fetoprotein (AFP) in eluates of dried-blood samples on filter paper by use of a simple, sensitive two-site enzyme immunoassay. The spot, 6 mm in diameter (equivalent to about 12 microL of whole blood), is incubated overnight with alkaline phosphatase conjugated to rabbit anti-AFP antibody in a tube containing a polystyrene bead coated with mouse monoclonal a...
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Serum cortisol level was measured by an enzyme immunoassay (EIA) using the enzyme penicillinase as a label without prior extraction and purification. Polyclonal antibodies were raised against cortisol-3-ortho-carboxymethyl-oxime (cortisol-3-0-CMO) conjugated to bovine serum albumin (BSA). This antibody showed a very low cross-reactivity with structurally related steroids (3.7% for corticos...
متن کاملHeterogeneous enzyme immunoassay of alpha-fetoprotein in maternal serum by flow-injection amperometric detection of 4-aminophenol.
A sandwich-type heterogeneous enzyme immunoassay with flow-injection analysis for alpha-fetoprotein (AFP) in human serum has been developed. 4-Aminophenol, the product of enzymatic reaction, is detected amperometrically. The interassay CV for this electrochemical enzyme immunoassay was less than 8.2%, with a minimum detection limit for AFP of 0.163 micrograms/L. The calibration curve had a line...
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Background: The maternal serum alpha-fetoprotein test (MSAFP) was developed to screen for neural tube defects. Little is known about the adoption of the MSAFP test. This study examines the effect of provider specialty and geographic location and patient insurance status on MSAFP test use in Washington State. Methods: We conducted a retrospective cohort study of MSAFP use in low-risk obstetric p...
متن کاملDetermination of tumor necrosis factor-alpha (TNF-alpha) in serum by a highly sensitive enzyme amplified lanthanide luminescence immunoassay.
OBJECTIVES To develop a highly sensitive enzyme amplified lanthanide luminescence (EALL) immunoassay for tumor necrosis factor-alpha (TNF-alpha). METHODS The method is based on the use of two monoclonal antibodies against TNF-alpha, one "capture" antibody and one labeled with biotin, in a "sandwich type" assay format. Alkaline phosphatase (ALP) conjugated to an antibiotin-polyclonal antibody ...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1982
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.30.4412